Dean's Undergraduate Research Awards
Johns Hopkins University
336 Garland Hall
3400 N. Charles Street
Baltimore, MD 21218
The structure-function paradigm i.e. that a protein must be in a unique conformation for function, has been challenged by the realized that a vast number of proteins are untrusted yet retain function. Interestingly, these intrinsically disorder proteins (IDPs) have hyper-abundance in cell-signaling pathways and transcription factors, where dysregulation can lead to many diseases including cancer. Therefore, developing techniques to determine the structure(s) of IDPs is of critical importance to improve medical therapeutic development. My project focuses on developing a novel technique to fold IDPs and probe for their structure using chemical cross-linking. Combining the rich information gathered from these experiments should provide us with information to predict the active conformation of IDPs and probe for dynamics of these structures. Insights gleaned from these studies have broad impacts for transcription factors involved in cancer.