Experimental and Computational Characterization of Novel SH3 Domain Proteins Designed Using Ensemble Information
SH3 domain containing proteins, such as the Ras oncogene product, have demonstrated importance in molecular signaling and cancer progression. To better understand its biological role, five completely new SH3 domain-like proteins have been engineered in this laboratory using a novel design algorithm. The algorithm models the SH3 domain’s thermodynamic ensemble, and fundamentally differs from state-of-the-art methods that rely on amino acid or structure similarity. Thus, this new technology may represent a breakthrough development for protein engineering. The proposed project will experimentally and computationally assess if the designs were successful. A scientific journal publication is the expected outcome of the project.
Mentor: Dr. Vincent Hilser, Department of Biology