Woodrow Wilson Undergraduate Research Fellowship Program
Johns Hopkins University
3400 N. Charles Street
Baltimore, MD 21218
JHU iGEM developed tools to control cellular processes in yeast, which can be applied to the optimization of non-native biosynthetic pathways used in cell-based manufacturing of compounds. These tools were an ethanol level self-regulation system, and a system for optogenetic control of protein function. For ethanol level regulation, the tool helped to decrease ethanol concentration under fermentation conditions without negatively impacting cell growth. The optogenetic control was used to control protein function in vivo and minimize stress on the host cell leading to maximization of the production of a desired compound.